In vitro Effect of Arnebia densiflora and Artemisia annua Plant Extracts against Theileria annulata Schizonts

Background: This study investigated the in vitro activities of naphthoquinones and artemisinin contained extracts obtained from Arnebia densiflora Artemisia annua plants against peripheral blood mononuclear cells infected with Theileria annulata schizonts. For this purpose, n-hexane extract A. petroleum ether methanol were obtained. Methods: Non-infected cultivated 2×106 seeded each well 0.5 ml RPMI 1640 medium. The was tested six times at concentrations 125, 62.5, 15.625, 7.8125 3.9062 mg/ml. also 31.25, 7.8125, 3.9062, 1.9531 mg/ml on PBMCs. A Mann-Whitney U test used to determine differences between control experimental groups. Result: killed both non-infected significant levels (P less than 0.05) compared group all concentrations. had no adverse effect insufficient, only observed a concentration 125 µg/ml. 15.625 µg/ml above 24 hours. At 48 hours, 62.5 In conclusion, can be future vivo studies for Theileriosis these effective